To generate IgG 1A5 D, containing the 9-aa deletion, two DraIII sites at positions 1053 and 1080 were 1st introduced to remove the intervening 27 nucleotides, and then the original splice site sequence was restored having a QuikChange Site-Directed Mutagenesis kit (Stratagene, La Jolla, CA)

To generate IgG 1A5 D, containing the 9-aa deletion, two DraIII sites at positions 1053 and 1080 were 1st introduced to remove the intervening 27 nucleotides, and then the original splice site sequence was restored having a QuikChange Site-Directed Mutagenesis … Continue reading To generate IgG 1A5 D, containing the 9-aa deletion, two DraIII sites at positions 1053 and 1080 were 1st introduced to remove the intervening 27 nucleotides, and then the original splice site sequence was restored having a QuikChange Site-Directed Mutagenesis kit (Stratagene, La Jolla, CA)